Abstract
Effect of Properties of Phosphate Buffer Saline Solutions on Vibrational Characteristics of a Quartz Crystal Microbalance Resonator
Highlights
PH buffers are commonly used in biochemistry to control pH and preserve protein stability
Until now, there has been no detailed report of the effect of the buffer solution on the oscillation characteristics of a quartz crystal microbalance (QCM) resonator. We experimentally investigate these effects by using a high-frequency QCM chip and a common phosphate buffer (PB) solution at various pH values, concentrations, and added salt contents
The frequency shift was calculated by subtracting the resonant frequency in water from the measured resonant frequency in the PB solution
Summary
PH buffers are commonly used in biochemistry to control pH and preserve protein stability. They are usually aqueous solutions of a weak acid (base) and its conjugated base (acid).(1) Salts are usually added to pH buffers to increase the ionic strength and thereby enhance buffering effectiveness. The buffer solution is usually used to establish a baseline for identifying signals produced by a bioreaction. The effect of the buffer solution on the transducer may not be neglected. Weng et al investigated the effect of the concentration of a phosphate buffer (PB) solution on an ion-sensitive field-effect transistor (ISFET)
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
