Effect of prom activated receptor-2 on severe acute pancreatitis

Abstract

Objective To investigate effect of protease activated receptor-2 (PAR-2) on pancreatic pathological damage in severe acute pancreatitis (SAP) in rats. Methods Experimental necrotic acute pancreatitis model was established by injection of 3.5% sodium taurocholate via the pan-creatic duct. A total of 36 rats were randomized into the control group, SAP group and pancreastitis with PAR-2 activating peptide group (PAR-2 group). All the rats were sacrificed 3 hours after injec-tion of sodium taurocholate. Immunohistoehemical analysis was used to test the expression of PAR-2 in pancreas before and after the induction of SAP. Histological score of pancreatic tissue specimen was assessed microscopically. Meanwhile, the ratio of pancreatic wet dry weight, the contents of my-eloperoxidase (MPO) and Evans blue leakage rate were determined. Interleukin-6 (IL-6) in serum was tested using ELISIA. Results PAR-2 was weakly expressed in cytoplasm and membrane before SAP was induced, and the expression increased after inducing of SAP. Activating PAR-2 significantly re-duced pancreatic histological score, MPO content, wet dry weight ratio and EB leakage rate. There was correlation between pancreatic histological score and IL-6. Conclusion The expression of PAR-2 increases after inducing of SAP. PAR-2 can reduce the severity of pancreatic inflammation and plays a protective role in the local pancreatic injury. Key words: Pancreatitis; Sodium taurocholate; Protease activated receptor-2