Effect of Probiotsid-Phyto and UDP Zn on the intestinal microbiome of Cyprinus carpio

Abstract

The system analysis of the phytobiotic “Probiocid®-Phyto” and zinc ultradispersed particles (Zn UDP) effect on growth dynamics, chemical composition of muscle tissue and structural microbiome of the common carp gut (Cyprinus carpio) was carried out in this article. The use of modern genetic methods of investigation (polymerase chain reaction with specific 16S prokaryotic primers and with Nextera XT protocol adapters and indices) allowed to determine that the main phylogenetic groups entering the intact group gut microbiome are Proteobacteria (40.49 %), Actinobacteria (28.67 %), Firmicutes (19.01 %) and Bacteroidetes (10.28 %). The most significant effect on the carp microbiome was exerted by Zn UDP, which was expressed by an increase in the specific weight of two types – Proteobacteria 66.64 % and Fusobacteria - 31.18 %, while in the bivalent application group the biodiversity of types was represented as follows: 57.08 % – Proteobacteria, 14.17 % – Firmicutes, 11.86 % – Actinobacteria, 7.85 % – Fusobacteria and 7.43 % – Bacteroidetes. The most significant effect of the preparations on the carp productivity was observed when the compared preparations were fed together; in this case, by the end of the experiment, the increase in carp live weight was 18.45 %. The research allows recommending the combined use of “Probiocid®- Phyto” (2 g/kg feed) and Zn UDP (10 mg/kg feed) as growth activators for common carp (Cyprinus carpio).