Effect of probiotic, prebiotic and symbiotic on toxicosis of ochratoxins in broilers

Abstract

his study was conducted to evaluate the prophylactic effect of multistrain probiotic in drinking water, prebiotic in diet and both of them (symbiotic) when afford with a diet containing ochratoxins and fed to broiler chicks from 1 to 35 days old. The criteria of evaluation include hematological and biochemical analysis, in addition to associated lesions. A total 150 Rose day old broiler chicks were separated into five groups that each one received different diets. Ochratoxicosis group showed significant (P<0.05) reduction in hematological parameters in comparison with probiotic, prebiotic, symbiotic and control groups. The biochemical analysis of ochratoxicosis group showed considerable (P<0.05) increase in the serum uric acid and creatinine levels with reduction in serum total protein, albumin and globulins. Pathological observations in kidney of broilers in groups 3, 4 and 5 revealed no pathological changes as compared with group 2 that showing necrosis of proximal renal tubules, severe congestion and lymphocytic proliferation. Spleen of ochratoxicosis group showing poor lymphoid tissue and depletion with necrosis of lymphocytes when compared with control and treated groups (probiotic, prebiotic and symbiotic) that appeared normal architecture represented by white and red pulp with mature lymphocytes, also there are germinal centers in symbiotic treated group. Normal thymus architecture in groups 3, 4 and 5 when compared with ochratoxicosis group that showed necrosis in the lymphoid follicles and hemorrhage. Finally, it was conducted that the probiotic, prebiotic and symbiotic are effective in the amelioration of toxic effects of ochratoxins. Symbiotic are more effect than probiotic or prebiotic alone.