Abstract
ABSTRACTBackground:Manipulating intestinal microbiota with probiotics might stimulate skin response. Understanding all stages of the healing process, as well as the gut-skin-healing response can improve the skin healing process. Aim:To evaluate the effect of perioperative oral administration of probiotics on the healing of skin wounds in rats. Methods:Seventy-two Wistar male adult rats were weighed and divided into two groups with 36 each, one control group (supplemented with oral maltodextrin 250 mg/day) and one probiotic group (supplemented with Lactobacillus paracasei LPC-37, Bifidobacterium lactis HN0019, Lactobacillus rhamnosus HN001, Lactobacillus acidophilus NCFM® at a dose of 250 mg/day), both given orally daily for 15 days. The two groups were subsequently divided into three subgroups according to the moment of euthanasia: in the 3rd, 7th and 10th postoperative days. Results:There were no significant changes in weight in both groups. Wound contraction was faster in probiotic group when compared to the controls, resulting in smaller wound area in the 7th postoperative day. As for histological aspects, the overall H&E score was lower in the probiotic group. The probiotic group showed increased fibrosis from 3rd to the 7th postoperative day. The type I collagen production was higher in the probiotic group at the 10th postoperative day, and the type III collagen increased in the 7th. Conclusion:The perioperative use of orally administrated probiotic was associated with a faster reduction of the wound area in rats probably by reducing the inflammatory phase, accelerating the fibrosis process and the deposition of collagen.
Highlights
The skin is a varied ecosystem composed of 1.8 m2 of tissue that covers the whole body, rich in folds, cutaneous attachments and contains a diverse microbiota[11]
The animal experiments were carried out in accordance with the norms established by the Brazilian Federal Law No 11,794, of October 8, 2008, Resolution 196/96 of the National Health Council, norms foreseen by the National Council of Control of Animal Experimentation (CONCEA) after approval of the Ethics Committee on the Use of Animals (CEUA) of the Positivo University (Opinion No 294)
The rats were weighed and divided into two groups with 36 animals each, one control which received maltodextrin 250 mg/ day) and one probiotic group supplemented with a probiotic Probiatop® from FQM-FARMA compound that contained Lactobacillus paracasei LPC-37, Bifidobacterium lactis HN0019, Lactobacillus rhamnosus HN001, Lactobacillus acidophilus NCFM®, at the dose of 250 mg/day, which corresponds to the approximate dose of 200,000 to 210,000 CFU, administered orally once a day, starting five days before surgery until the euthanasia day, with the aid of a spatula[24] mixed in cream cheese
Summary
The skin is a varied ecosystem composed of 1.8 m2 of tissue that covers the whole body, rich in folds, cutaneous attachments and contains a diverse microbiota[11]. Advanced molecular analyses of the cutaneous microbiota revealed a great diversity and these vary according to its topographic location on the body[7]. Healing is a dynamic cellular process involving molecular and biochemical events aimed at tissue reconstitution[3,4]. The microbiota of the skin plays a key role in the immunological response and can interfere in the wound healing[20]. The perception of the skin as an ecosystem rich in living biological components and present in different locations explains the delicate balance between host and microorganisms. The skin microbiota is influenced by the intestinal
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