Effect of probiotic on larvae shrimp ( Penaeus vannamei) based on water quality, survival rate and digestive enzyme activities

Abstract

The effect of probiotic, B. coagulans SC8168, as water additive on larvae shrimp ( Penaeus vannamei) based on water quality, survival rate and digestive enzyme activities was investigated at ontogenetic stages (Z 3, M 3, PL 1–2 and PL 7–8). Twelve tanks with three replicates for each treatment group and control group were used. The treatments consisted of three SC8168 levels at an initial concentration of 1.0 × 10 5 cfu ml − 1 (T-1), 5.0 × 10 5 cfu ml − 1 (T-2) and 1.0 × 10 6 cfu ml − 1 (T-3) and one control (without any probiotic), and were conducted every day. Addition of the probiotic significantly increased survival rate ( P < 0.05) for all treatments over controls. However, no significant difference was found between T-2 and T-3. At early larval stages (Z 3 and M 3), protease activity in shrimp was not significantly different among probiotic treatments and control. At the subsequent ontogenetic stages (PL 1–2 and PL 7–8), the highest protease activity was observed in T-2 and there was a significant difference ( P < 0.05) between the treatment and the control. Similar results were observed in T-3 at PL 7–8 stage ( P < 0.05). Amylase activity in T-2 at Z 3, M 3, PL 1–2 and PL 7–8 stages was significantly higher ( P < 0.05) than that in the control. The amylase activity was also increased significantly ( P < 0.05) in T-3 than the control except the M 3 stage. As for the lipase activity, assays showed a significant difference ( P < 0.05) in groups treated with SC8168 as compared with the control except the initial stage (Z 3). However, a concentration response of probiotic strains in T-1, T-2 and T-3 was not observed in the present research. The results from this study suggest that B. coagulans SC8168 supplemented at a certain concentration could significantly increase survival rate and some digestive enzyme activities of P. vannamei larvae.