​Effect of Probiotic Enterococcus gallinarum N3 Supplemented Feed on Growth Performance of Freshwater Fish Cyprinus carpio

Abstract

Background: Probiotic is the best solution (Produce antimicrobial compounds, inhibit colonization of pathogenic microorganism, increase digestive ability and confer better health to the host) as an alternative to antibiotics against various microbial infection in freshwater and marine water. The freshwater fish Cyprinus carpio was selected for this research work (due to high growth rate, high protein content, ability to survive under different climatic conditions and its availability throughout the year) to observe the effect of probiotic Enterococcus gallinarum N3 supplemented feed on its growth. Methods: In this present research work Prepared three different concentration of probiotic Enterococcus gallinarum N3 (0.1%. 0.5% and 1.0%) along with basal diet containing other ingredients (fish meal, groundnut oil cake, wheat bran, starch and Vitamin mineral premix). Initially 14 days acclimatized freshwater fish Cyprinus carpio to laboratory condition selected randomly for four treatment groups as Control (C) and probiotic feed treated T1, T2 and T3 group. The experiment was carried out for 42 days with continuous aeration, natural photoperiod of 16 hrs light and 8 hrs dark, maintain temperature constant and fishes fed diet twice daily. The feed ingredients and experimental diets analysed for proximate composition and measured Weight gain, Specific growth rate (SGR), and Feed conversion efficiency (FCE). Result: The proximate composition of the feed was determined as percentage dry matter in which 36.21% protein, 6.8% moisture and 7.8% of lipid. The probiotic feed experiment was conducted for 42 days and entire group of fishes was survived. The effect of probiotic feed on growth performance of Cyprinus carpio was measured as initial weight, final weight, weight gain, specific growth rate and feed conversion efficiency. The growth changes were observed before and after treatment with 0.1%, 0.5% and 1.0% of probiotic E. gallinarum N3 fed to Cyprinus carpio. The final weight (g) and final length (cm) was measured in treatment T1 (3.107 g; 3.32 cm), T2 (3.258 g; 3.40 cm) and T3 (3.413 g; 3.52 cm) respectively as compared with Control group C (2.255 g; 2.98 cm). The percentage weight gain obtained in group T1 (37.78%), T2 (44.45%) and (51.55%) respectively as compared with Control group C (29.54%). Maximum specific growth rate was observed in treatments T1 (0.94 % day-1), T2 (0.99% day-1) and T3 (1.02% day-1) as compared with control group C (0.56% day-1). The results showed better growth performance and feed utilization in all the three treated groups T1, T2 and T3. The best growth performance (final weight, final length, weight gain, specific growth rate and feed conversion efficiency) and feed utilization (moisture, protein and lipid) was observed best in T3 (1 g kg-1) group of fishes as compared to control C group. There was significant difference (P greater than 0.05) in final weight, final length, weight gain, specific growth rate and feed conversion efficiency.