Abstract
This study was conducted to evaluate pretreatment processes to obtain the lightest color using the minimum total color difference (ΔE*), the maximum solubility point, and the highest gelatin recovery for gelatin production from rainbow trout skin. The hydroxyproline content of the gelatin for the sample giving the highest hydroxyproline/protein ratio was 4.64%, which was defined as pure gelatin. The purity of the other samples were between 72.1 and 98% (p < 0.01). The effect of the pretreatment variables NaOH concentration (0.01–0.21 N), acetic acid concentration (0.01–0.21 N), and combined alkali/acidic pretreatment time (1–3 h) were studied using a central composite rotatable design with the equal weighting of the three dependent factors to model the system. Response surface methodology was used for optimization. According to the model, optimum extraction conditions were found to be 0.148 N OH−, 0.181 N H+, and 2.71-h pretreatment time giving a predicted minimum solubility at pH 8.44, a ΔE* of 12, and 84.3% gelatin recovery. In addition, the pretreatment time was confirmed as being the most significant factor affecting all responses along with the alkali pretreatment solutions which were also considered significant for gelatin recovery.
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