Effect of pressure on the bilayer phase transitions of asymmetric lipids with an unsaturated acyl chain in sn-1 position

Abstract

The bilayer phase transitions of a series of mixed-chain phosphatidylcholines (PCs) with an unsaturated chain in the sn-1 position and saturated chain with different lengths in the sn-2 position, 1-oleoyl-2-myristoyl-PC (OMPC), 1-oleoyl-2-palmitoyl-PC (OPPC) and 1-oleoyl-2-stearoyl-PC (OSPC), were observed by differential scanning calorimetry (DSC) under ambient pressure and light transmittance measurements under high pressure. All the PC bilayer membranes exhibited not only the main transition from the lamellar gel (Lβ) phase to the liquid crystalline (Lα) phase but also the transition from the lamellar crystal (Lc) to the Lβ (or Lα) phase. The thermodynamic quantities of the transition between the Lc and Lα phases for bilayers of unsaturated mixed-chain PCs were compared with those of the corresponding mixed-chain PCs with saturated acyl chains such as 1-stearoyl-2-myristoyl-PC (SMPC), 1-stearoyl-2-palmitoyl-PC (SPPC) and 1,2-distearoyl-PC (DSPC). It turned out that the partial molar quantities of the unsaturated mixed-chain PC bilayers in the Lc phase are larger than those of the saturated mixed-chain PCs bilayers. The increase in the partial molar quantities in the Lc phase by the introduction of a cis double bond into the sn-1 acyl chain is attributable to the loose packing of the saturated and unsaturated acyl chains in the Lc phase.