Abstract
This study investigated the effect of temperature and methane partial pressure on the anaerobic methane-oxidizing and sulfate-reducing (AOM-SR) activities by a highly enriched ANME-2a community. The ANME-2a-enriched biomass was incubated at different pressures, i.e., 2, 10, 20, and 30MPa at 15°C for 80days. The response of the microbial community with temperature was investigated in incubations at 4, 15, and 25°C at 10MPa. Among all tested conditions, the incubation at 10MPa pressure and 15°C showed the highest AOM-SR activity of the studied ANME-2a phylotype, whereas activity at 2MPa pressure and 15°C was almost comparative to the response at 10MPa pressure. The finding of the most favorable conditions for AOM-SR activity by the studied AOM-SR community comparable to the in situ pressure and temperature (15°C at 10MPa) suggests that the studied ANME-2a phylotype was well adapted to the conditions similar to its origin. The microbial community analysis showed that the bacterial community composition shifted upon changing the incubation temperature and pressure.
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