Abstract
ABSTRACTCrude calcium‐activated factor (CAF) was prepared from pre‐rigor pressurized (PRP) and corresponding control (CON) bovine muscles at 0, 1, and 3 days of postmortem times. Throughout these periods, PRP samples had consistently lower total CAF activity than CON. Yet, it seems probable that PRP caused a transient increase in Ca++ sufficient to activate CAF for a brief period of time because (1) early postmortem, PRP muscle had quantities of a 95,000‐dalton component presumably produced by CAF; (2) Z‐lines from at‐death PRP muscles were highly degraded; (3) very early postmortem storage, the SDS‐electrophoretic patterns of PRP myofibrils resembled that of CAF‐treated myotibrils. CAF autolysis may account for the low CAF activity observed in PRP muscles at later postmortem storage times.
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