Effect of Prenatal Ethanol and Stress on Levels of β‐Endorphin in Different Brain Regions of the Rat

Abstract

The combination of prenatal ethanol exposure and footshock stress was investigated for its effects on brain beta-endorphin levels. Subjects were offspring of rats that received 1 of 3 prenatal dietary treatments: an ethanol-containing liquid diet, a identical liquid diet with ethanol substituted isocalorically with maltose-dextrin (pair-fed group), and standard laboratory rat chow (chow-fed group). Two different stress paradigms were used: a short (30-sec) footshock stress paradigm and a prolonged (180-sec) footshock stress paradigm. Levels of beta-endorphin were measured with radioimmunoassay in eight brain regions of unstressed (baseline) rats, and of stressed rats at 3 and 30 min following termination of the stress. Seven brain regions containing high densities of beta-endorphin axons and terminals were chosen, as well as the arcuate region of the hypothalamus, the only brain region where both beta-endorphin perikarya and terminals are located. Following the short footshock stress paradigm, there were no changes in beta-endorphin levels, except for a trend toward increased levels in the pair-fed group. After the prolonged stress paradigm, levels of beta-endorphin in both the pair-fed and chow-fed groups tended to be decreased in several brain regions, including the arcuate region, at 3 min after termination of the stress. In contrast, for the prenatally ethanol-exposed group, beta-endorphin levels were increased significantly in the arcuate region, and moderately increased in the septal/preoptic region and medulla/pons at 3 min after the prolonged stress paradigm.(ABSTRACT TRUNCATED AT 250 WORDS)