Abstract
1. 1. Effect of stage of pregnancy and lactation on levels of protein. DNA, lactose synthetase. CMP- N-acetylneuraminic acid synthetase, CMP- N-acetylneuraminic acid: GM 3 sialyltransferase. CMP- N-acetylneuraminic acid: GM, sialyltransferase. and UDP-galactose:glucosyl ceramide galactosyltransferase in rat mammary gland were examined. 2. 2. In all cases protein content and enzyme activities began to increase one to 2 weeks before parturition and demonstrated sharp increases one to three days after parturition. 3. 3. Maximum enzyme activity, expressed per unit protein, occurred around day thirteen of lactation for all enzymes examined except for CMP- N-acetylneuraminic acid: GM 3 sialyltransferase. 4. 4. There was a strong positive correlation between the levels of lactose synthetase. CMP- N-acetylneuraminic acid synthetase. CMP- N-acetylneuraminic acid: GM 1 sialyltransferase and UDP-galactose:glucosyl ceramide galactosyl-transferase on any given day. 5. 5. Analysis of variance demonstrated that enzyme levels showed linear, quadratic and cubic properties over the course of the experimental period.
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