Abstract
Human oocytes have been successfully cryopreserved using both conventional freezing and vitrification protocols. However, regardless of the method used, post-thaw results have been inconsistent and disappointing, limiting clinical application of oocyte cryopreservation. We examined the effect of pre-vitrification oocyte zona slitting, temperature variation and the effect of two different cryoprotectants (DMSO and EG) on post-warmed oocyte viability, subsequent fertilization potential and blastocyst development.
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