Abstract
In this study, the effect of five pre-treatments (thermal, ultrasound, acid, base and bacterial product) on hydrolysis and methane production potentials of four by-products from meat-processing industry was studied. The bacterial product Liquid Certizyme 5™ increased soluble chemical oxygen demand (CODsol) of digestive tract content and drumsieve waste the most as compared to untreated material (62 and 96%, respectively), while ultrasound was the most effective to increase CODsol with dissolved air flotation (DAF) sludge (88%) and grease trap sludge (188%). In batch experiments, thermal treatment increased methane production potential of drumsieve waste, acid of grease trap sludge and all pre-treatments of DAF sludge. However, with all other pre-treatments, methane production potential was decreased compared to untreated materials, apparently due to inhibition by hydrolysis products and/or possible re-crystallization of some compounds. Methane production potentials from the untreated materials were as follows: digestive tract content 400 ± 50 m 3 CH 4/t volatile solids (VS) added, drumsieve waste 230 ± 20 m 3 CH 4/tVS added, DAF sludge 340 ± 17 m 3 CH 4/tVS added and grease trap sludge 900 ± 44 m 3 CH 4/tVS added.
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