Abstract
BackgroundSamples for diagnostic procedures often require some form of pre-analytical preparation for preservation or safe handling during transportation prior to analysis in the laboratory. This is particularly important for milk samples which frequently need preservatives to retain milk composition as close to that found in freshly collected samples as possible.MethodsMilk samples were treated by heating at 56 °C for 30 min or preserved by addition of either potassium dichromate or bronopol respectively. Haptoglobin (Hp), mammary associated serum amyloid A3 (M-SAA3) and C-reactive protein (CRP) were measured in the various treatment groups and in control samples which were not treated, using enzyme linked immunoassays. The concentrations of each APP were compared between treated and non-treated groups using the Wilcoxon signed ranks tests.ResultsHeat treatment of samples was found to have a significant lowering effect on milk M-SAA3 and CRP but not Hp. The use of potassium dichromate and bronopol as preservatives in milk had no significant effects on milk Hp and M-SAA3 concentration but lowered milk CRP values compared to controls.ConclusionsThe observed effects of heating and preservative use on milk APP should be taken into consideration when assaying samples which have undergone heat treatment as a result of international transfer regulations involving biological samples or samples needing chemical preservation prior to transport to laboratory.
Highlights
Samples for diagnostic procedures often require some form of pre-analytical preparation for preservation or safe handling during transportation prior to analysis in the laboratory
Given that bovine mastitis is one of the most prevalent and costly conditions affecting dairy cows which greatly impacts on the economics, welfare and public health aspects of dairy farming, the substantiation of acute phase proteins (APP) for mastitis diagnosis is on-going and methods to apply their measurement to a rapid format has been explored [3, 4]
This is especially true for milk samples which frequently need the use of preservatives to retain milk composition as close to that found in freshly collected samples as possible
Summary
Samples for diagnostic procedures often require some form of pre-analytical preparation for preservation or safe handling during transportation prior to analysis in the laboratory This is important for milk samples which frequently need preservatives to retain milk composition as close to that found in freshly collected samples as possible. Acute phase proteins are proteins that are synthesized in the liver and other organs in response to inflammatory stimuli and released into body fluids such as serum, milk [1], ovarian fluid [5] among others These samples are collected and assayed for APP in order to make clinical diagnosis of inflammatory and other disease conditions. Storage at freezing temperatures of between −20 °C and −80 °C is a common way of preserving milk samples
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