Abstract
The aim of the present paper was to analyze the influence of sampling and storage procedures on nitrite con- centration values in the saliva of healthy persons. The samples were obtained and stored under varied condi- tions, and processed using the Griess method. Results: when the salivary nitrite concentration was measured immediately after collection a significant dependence on the collection time was observed. A mean value of 94 µmol/L (range 3 - 625) was obtained at 8:30 am. This value decreased significantly with time (p < 0.05) reaching a value of 68 at 12:30 noon. Concerning the sample storage, a significant increase in the nitrite concentration was observed after 2 hrs, either at 4?C or at room temperature (p < 0.05). In spite of the high variability between individuals the values for each individual showed a marked constancy independent of the sampling day. According to our results, by controlling pre-analytical parameters, principally sampling and storage procedures, reproducibility is improved.
Highlights
N-nitroso compounds have been shown to be potent carcinogens in animals [1]; in the human body they can be formed by the interaction of nitrite and a variety of amine precursors, developing infantile methahemoglobinemia and gastric cancer
The aim of the present paper was to analyze the influence of sampling and storage procedures on nitrite concentration values in the saliva of healthy persons
In the present study we analyze the variations of salivary nitrite in healthy people before breakfast during the morning and those related to the storage conditions, in order to obtain reliable sampling for further clinical studies
Summary
N-nitroso compounds have been shown to be potent carcinogens in animals [1]; in the human body they can be formed by the interaction of nitrite and a variety of amine precursors, developing infantile methahemoglobinemia and gastric cancer. Nitrite has been extensively studied in relation to carcinogenesis [2]; it is an important factor for gastric nitrozation, and could contribute to the etiology of lung, stomach, esophagus, nasal cavity, bladder and oral cavity cancer, leukemia and Non Hodgkin lymphoma [3]. It has mutagenic effects at cellular levels and acts on p53 gene, closely related to the head and neck squamous cell carcinoma [4]. It has been estimated that 70% of the orally ingested nitrate is reduced to nitrite by mouth microorganisms, mainly on the posterior surface of the tongue [9,10]
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