Effect of potassium phenosan on structure of plasma membranes of mice liver cells in vitro

Abstract

The effect of synthetic anti-oxidant potassium phenosan (PP, potassium salt of beta-(4-hydroxy-3,5-ditretbutil-phenyl)-propionic acid) on the structural state of the surface (8 angstroms) and deep (20-22 angstroms) lipid regions of plasma membranes of mice liver cells was studied by spin probes method in vitro in a wide range of concentrations (1(-5)-10(-21) M). Two stable free radicals, 5- and 16-redox-stearic acids (C5 and C16), were used as spin probes. The nonlinear polymodal dose-effect dependences were obtained for parameters that characterize the microviscosity of the lipid bilayer (tau(c)) in the site of localization of the probe C16, and the order parameter (S), which characterizes the stiffness of the surface layers of lipids in the site of localization of the probe C5. Statistically a reliable increase was observed for parameter tau(c) after addition of PP at concentrations 10(-5)-10(-7) M and 10(-18)-10(-19) M, and for parameter S after addition of PP at concentrations 10(-6)-10(-7) M and 10(-13)-10(-15) M. Peaks on both dose-effect curves were separated by the intervals of concentrations where PP had no effect on the studied physico-chemical characteristics of biomembranes. For PP concentrations which caused maximal changes in tau(c) and S, we investigated thermal dependence of these parameters and determined the thermally induced structural transitions. Comparing with control, ultra-low doses of PP (10(-13)-10(-15) M) and (10(-18)-10(-19) M) caused an appearance of additional thermally induced structural transition in the surface and deep regions of plasma membrane lipids. The possible role of the interaction of PP molecules with specific binding sites on plasma membranes and formation of nanoparticles of PP in very dilute aqueous solutions are discussed.