Abstract
Increased potassium ion concentration in the incubating medium (80 mM) resulted in the enhancement of oxygen consumption, glucose utilization and lactate production in excised superior cervical sympathetic ganglia from rats. But the enhancement was less in the ganglion preparation than in brain cortex slices. In axotomized ganglia, which were prepared by cutting surgically the postganglionic nerve fibers in vivo, little change in oxygen uptake was shown, but there was a considerable increase in glucose utilization and lactate formation as compared with the normal control ganglia. In axotomized ganglia, 32P-incorporation into phospholipids such as phosphatidyl inositol, phosphatidyl choline, phosphatidyl ethanolamine and phosphatidic acid was reduced nearly to a half of that in the uncut control. With increased potassium ion concentration, 32P-incorporation into phospholipids in axotomized ganglia was restored, particularly for phosphatidyl inositol and phosphatidyl choline. Addition of amino acids to the incubation medium did not affect the 32P-turnover of the excised ganglia with or without axotomy, except in the case of GABA. Possible roles of not only neuronal but also non-neuronal elements in aerobic glucose and phospholipid metabolism and their relationship to ionic and amino acid transport mechanisms are discussed.
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