Effect of post-silking drought stress on the expression profiles of genes involved in carbon and nitrogen metabolism during leaf senescence in maize (Zea mays L.)

Abstract

Drought stress during reproductive growth stages greatly affects the growth and productivity of maize plants. To better understand the metabolic regulation during post-silking drought (PD) stress, an RNA sequencing (RNA-Seq) analysis was performed at the late stage of leaf senescence in maize. Physiological measurements showed that PD stress reduced both leaf carbon and nitrogen levels. A total of 4013 differentially expressed genes (DEGs) were found based on RNA-Seq analysis, 115 of which were identified to be involved in photosynthesis and in the metabolism of sucrose, starch, and amino acids. Among these DEGs, 14 genes involved in photosynthesis were down-regulated. The genes coding for sucrose and pectin synthesis were up-regulated under PD stress. The two genes of asparagine synthetase (ZmAS3 and ZmAS4), which are responsible for nitrogen remobilization in leaves, were also significantly induced by the drought treatment. The expression profiles of these genes involved in carbon and nitrogen metabolism suggests their regulatory roles during drought-induced leaf senescence.