Effect of post-thaw supplementation of seminal plasma to the cryopreserved spermatozoa of Malabari bucks on in vivo fertility

Abstract

The study was conducted to assess the effect of post-thaw supplementation of seminal plasma to the cryopreserved spermatozoa of Malabari bucks on in vivo fertility. The ejaculates collected from Malabari bucks of good (n=3) and poor (n=3) semen freezability were pooled separately and cryopreserved. After thawing, seminal plasma from good semen freezability bucks was supplemented in such a way that group I (GFSP) included thawed semen of good freezability with supplementation, group II (GF) included thawed semen of good freezability without supplementation, group III (PFSP) included thawed semen of poor freezability with supplementation and group IV (PF) included thawed semen of poor freezability without supplementation. After a period of incubation for 10 min. at 37°C, the semen from each group was used for transcervical insemination of Malabari does of proven fertility. The results showed that there was a significant improvement in the conception rate with post-thaw supplementation. Percent of animals kidded also differed significantly in the four groups. But, prolificacy did not differ in GF and GFSP.