Abstract
The effect of the addition of polyvinylpyrrolidone (PVP-40) to grapevine protoplast cultures ( Vitis sp. cv. Vidal blanc) at different stages of development (day 0, 7 and 14 of cultivation) and the influence of activated charcoal (AC) in several concentrations (0, 0.1, 0.5 and 1.0 %) on protoplast growth were studied. Both additives were investigated using different modified culture media (CPW-13, V/KM, MS-P). The application of 0.5% PVP could not prevent browning of the culture media but reduced it to a low level. Although the effect of PVP on plating efficiency was not clear, the formation of microcalli from grapevine protoplasts was remarkably improved when adding PVP at ''day 0'' or ''day 7'' of cultivation. AC could prevent browning of the media in all concentrations tested but at the same time inhibited plating efficiency. Conversely, formation of microalli occurred only with 0.5 and 1.0% AC, but at very low frequencies. A superior suitability of one of the culture media tested for grapevine protoplasts culture could not been found.
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