Effect of Polystyrene Targeting Nanoparticles on Lung Injury in Severe Acute Pancreatitis and NOX2/ROS/NF-κB Pathway

Abstract

Relationship between polyethylene targeting nanoparticles and key components of the NOX2/ROS/NF-κB signaling pathway has not yet been fully clarified, and their regulatory role in lung injury in severe acute pancreatitis has not yet been confirmed. In this study, severe acute pancreatitis lung injury cells were exposed to polyethylene targeting nanoparticles and MTT method was used to detect cell proliferation. Cell cycle and apoptosis rate were detected using flow cytometry and the expression of NOX2/ROS/NF-κB pathway was detected. The compound polyethylene targeting nanoparticles inhibited proliferation of lung-damaged cells in severe acute pancreatitis dose-dependently (5, 10 and 20 μmol/L), induced G2/M phase arrest, and increased cell apoptosis. In addition, it reduced the expression of NOX2, ROS, and NF-κB, indicating that NOX2/ROS/NF-κB pathway may be inhibited. Polystyrene targeting nanoparticles reduced the expression of IL-6, TNF-α, JAK, STAT, and IL-10. As a targeted drug delivery system, nano-drug-carrying systems help to dissolve drugs that are difficult to dissolve in the drug solution and intervene in the corresponding tissues and cells in a targeted manner. The results of this study showed that polymer-targeted nano-drug systems could regulate the growth of lung-damaged cells in severe acute pancreatitis. Polyethylene targeting nanoparticles may be effective in inhibiting inflammation in lung-damaged cells in severe acute pancreatitis via regulation of NOX2/ROS/NF-κB pathway.