Effect of Polysaccharides from Bletilla striata on the Healing of Dermal Wounds in Mice.

Chen Zhang,Zhejie Chen,Jinming Zhang,Yanan He,Jinfeng Shi,Yan Qu

doi:10.1155/2019/9212314

Abstract

Bletilla striata has been largely used in traditional folk medicine in China as a wound healing agent and to treat gastritis and several other health problems. Some studies have shown that plant polysaccharides may have the ability to promote wound healing. The aim of this work was to evaluate the wound healing activity of the polysaccharide extracted from Bletilla striata. Firstly, a Bletilla striata polysaccharide was extracted by water extraction and alcohol precipitation and characterized by Fourier transform infrared spectroscopy. The Bletilla striata polysaccharide was then tested for cell migration and proliferation using the mouse fibroblast cell line. Then, the Bletilla striata hydrogel was fabricated for acute wound health care of the mouse full-thickness excision. The results showed that the BSP enhanced the proliferation and migration of L929 cells. The superior wound healing capacity of the BSP hydrogel was demonstrated that it significantly accelerated the wound healing process in vivo in full-thickness skin defect wounded models. Compared to the saline group, the BSP hydrogel could accelerate wound healing and promote re-epithelialization and collagen deposition by means of TGF-β/Smad signal pathway activation. Taken together, BSP hydrogel would be a useful pharmaceutic candidate for acute cutaneous wound health care.

Highlights

Wound healing involves four overlapping phases of coagulation, inflammation, proliferation, and tissue remodeling and is a dynamic and complex process controlled by many factors [1]. e way and time of wound healing depends on the degree of injury, tissue regeneration ability, necrotic tissue, foreign body infection, and so on [2]
The aqueous phase was successively collected to repeat the Sevag deproteinisation process until no obvious denatured protein precipitate was found in the n-butanol interlayer. e deproteinated aqueous fraction was dialyzed against the membrane with molecular weight cut-off (MWCO) of 5 KDa. e resultant liquor was precipitated with 3 times the volume of 95% ethanol and placed in 4°C for 24 h
scanning electron microscopy (SEM) was used to evaluate the topographic characteristics and morphology of the Bletilla striata polysaccharide (BSP) hydrogel as they are related to swelling, dissolution, and release characteristics

Summary

Introduction

Wound healing involves four overlapping phases of coagulation, inflammation, proliferation, and tissue remodeling and is a dynamic and complex process controlled by many factors [1]. e way and time of wound healing depends on the degree of injury, tissue regeneration ability, necrotic tissue, foreign body infection, and so on [2]. Natural products from plants and marine lives, as an alternative source of drugs which modulate the inflammatory process in a shorter time period and promote skin tissue regeneration in the treatment of skin lesions, have become a popular topic of scientific research, such as honey [6], propolis [7], marine fungus [8], squid ink polysaccharide [9], and so on. Ese natural products are believed to offer effective, affordable, and accessible forms of treatment Among these natural products, polysaccharides have attracted widespread attention and have been demonstrated to promote skin tissue repair on the healing of dermal wounds by ameliorating oxidative stress, inflammation, and secondary trauma [10]. The BSP has great potential and application prospects in biological delivery systems, wound dressings, and other biomaterials because of its great

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Conclusion