Effect of polymyxin B on intestinal bacterial translocation in Pseudomonas aeruginosa wound-colonized burned mice.

Abstract

Bacterial translocation (BT) from the gastrointestinal tract has been proposed to play a role in the pathogenesis of septic complications in severely burned patients. In a burn model the effect of a subtherapeutic dose of polymyxin B-sulfate (PB) at BT was examined in Escherichia coli-monoassociated mice with Pseudomonas aeruginosa-inoculated burn wounds. The BT incidence and number of translocating microorganisms to the spleen (p less than 0.01), liver (p less than 0.01), lung (p less than 0.05) and heart (p less than 0.05) were diminished significantly in the PB-treated versus the untreated group. Endotoxin in plasma was detectable in one of the 16 PB-treated versus 6 of the 17 control mice (p less than 0.05). The relation of Pseudomonas burn wound inoculation, BT, endotoxin and the endotoxin-neutralizing properties of PB will be discussed.