Effect of polymer oil drive process in Daqing oil field on the microorganism community in oil deposit system

Abstract

By analyzing the composition of pumped water from polymer oil drive process in Daqing Oil Field, measuring total DNA of pumped liquid of typical polymer oil drive process and constructing bacterium 16S rDNA clones tree, effect of oil drive process and chemicals used in three oil recovery technique of ternary compound oil drive on microorganism community, heredity variety and the environment was preliminarily investigated based on restriction fragment length polymorphism(RFLP) analysis and sequencing. The results show that the bacterium of pumped liquid belong to seven main dominant monoid of operational taxonomic unit, mainly distributing in the following three fungi gates: Proteobacteriaδ、β、e、γ(5.71%、8.57%、28.57%、28.57% respectively), Firimicutes Clostridia(25.71%), (Deferribacteres)(2.86%). The Proteobacteria makes up of 73% of the total clones. Hydrocarbon seep bacterium, Arcobacter sp .and Clostridium cylindrosporum are dominant spawns. System development analysis illustrated that, the microbe resource of oil deposit system is new, properly belonging to new species, and most of microbe maybe do not come from this system. The using of polymer solution and ternary compound oil drive chemicals is the main factor determining microbe distribution in the oil deposition system, which results in the decreasing of the microbe population variety of oil deposit system.