EFFECT OF POLYHERBAL FORMULATION OF Boerhavia diffusa, Sesbania grandiflora, Moringa olifera, AND Solanum nigrum AGAINST GASTROENTERITIS AND ITS VALIDATION USING In silico APPROACH

Abstract

Boerhavia diffusa, Sesbania grandiflora, Moringa olifera, and Solanum nigrum are indigenous plants that have been used in traditional medicine for treating various gastroenteritis. The present study aimed to demonstrate the Acid-neutralizing capacity, invitro anti-urease, anti-oxidant, inhibition of lipid peroxidation ability, anti- Inflammatory, and H+/K+ ATPase inhibition activity of hydroalcoholic extract of the polyherbal formulation. The phytochemicals present in the polyherbal formulation (PHF) were identified by GC – MS analysis. The total Phenolic Content and total Flavonoid content of phytochemicals were quantified. Phytochemical interactions with the active sites of urease were supported by Molecular docking analysis. The results revealed that 0.02 mg of phenolic content and 0.013 mg of flavonoids per gram of plant extract were observed, PHF had acid- neutralizing capacity better than the standard antacid tablet, 38% of anti-urease activity towards urease enzyme, 64% of inhibition of lipid peroxidation against oxidation of lipid bilayer, 59% of inhibition of protein denaturation, 37% of haemolysis inhibition, 80% of H+/K+ ATPase inhibition activity in the goat parietal cells. There were 14 functional compounds tend to interact with the enzyme urease. But two compounds directly interacted with the active site of ureases.