Abstract
The aim of this study was to evaluate the effect of anti-tumor necrosis factor α (TNFα) antibodies on the TNFα gene expression in a neonatal septic shock model. Ten-day-old Sprague-Dawley rats were divided into four groups and given intraperitoneal (ip) injection as follows: group 1: 0.1 ml saline; group 2: 0.1 mg/kg Salmonella enteritidis lipopolysaccharide (LPS); group 3: 1 mg/kg of anti-TNFα antibodies (Ab); group 4: 0.1 mg/kg of LPS and 1 mg/kg of Ab. We found that in group 2, LPS induced shock, demonstrating hypoglycemia and lactacidemia (p < 0.05) and death (81.8%). Ab decreased the mortality significantly (35%) and attenuated the hypoglycemia (35 ± 8 mg/dl in group 2 vs. 53 ± 3 mg/dl in group 4) and lactacidemia (5.40 ± 0.63 vs. 2.35 ± 0.45 mmol) at 8 h in group 4 when compared to group 2. Northern blot demonstrated a significant decrease in TNFα mRNA expression in group 4 as compared to group 2, at 2 h after LPS injection. We conclude that the beneficial effects of anti-TNFα antibodies on LPS-induced shock may be due to decreased TNFα gene expression.
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