Abstract
1. The effect of polyamines (spermine, spermidine and putrescine) on rat liver cell-free polypeptide synthesis was studied in the following three systems: (1) microsome-S100 fraction system, (2) polysome-S100 fraction system and (3) ribosome-poly(U)-S100 fraction system. 2. In the presence of Mg 2+ of sub-optimal concentration, the polypeptide synthesis was stimulated by polyamines in all three systems. The maximum incorporation of radioactivity into the polypeptide fraction under these conditions was almost equal to that obtained at optimal Mg 2+ concentration without polyamines. 3. In the presence of very low Mg 2+, no significant level of polypeptide synthesis was observed regardless of the amount of polyamines added, thus showing that the sparing effect of polyamines on Mg 2+ in rat liver polypeptide synthesis is only partial. 4. Putrescine (at all concentrations tested) and spermidine (less than 1.0 mM) stimulated polypeptide synthesis even at optimal Mg 2+ concentration when polysomes were attached to the endoplasmic reticulum.
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