Effect of polyamines and zeolites on the protein profile of leaves of the date palm cuttings Phoenix dactylifera L. grown under heavy metal stress conditions

Abstract

This study was conducted on the five to six years old offshoots of date palm (Jebjab) cultivar planted in sustainable land, using an environment contaminated with heavy elements, with irrigation water contaminated with heavy elements Pb(NO3)2 300 mg.kg-1 and cadmium chloride CdCl2 3 mmol, as well as the comparison treatment. Then the treatment was with some polyamine compounds Putrescine 500 mg L-1, coumaric acid 500 mg L-1, and a zeolite compound at a rate of 10 kg Palm-1, in addition to the comparison treatment in the form of ground addition to the soil of the off shoots samples were taken from palm fronds, samples were dried using Freeze-dryer (Lyophilization technique), the protein was extracted from samples, and protein migration was carried out on Polyacrylamide gel using Slab-Electrophoresis method in the presence of SDS denaturants. The molecular weights of proteins were estimated and plotted using PhotoCapt Mw. The results of the study showed that the protein pattern of the leaves show up that all the trees of the study had taken part (identical) with the first protein bundle and the fifth protein bundle on the polyacrylamide gel, as the molecular weights of the proteins of the first bundle ranged between (222.136-243.750) kDa, while the molecular weights of the proteins of the bundle ranged. The fifth is between (24.843-50.386) kDa. This coincidence or participation in the molecular weights of the protein bundles of all the study trees may indicate that these trees belong to one plant origin (which is the Jebjab variety), as these plants share close molecular weights. Keywords: Date Palm, molecular weights, polyacrylamide gel, polyamine compounds.