Abstract
Objective: This study was conducted to evaluate the influence of pollution with lead, copper, cadmium on the gene expression pattern of liver Glutathione-S-transferase and serum lysozyme in Nile tilapia (Oreochromis. niloticus). Design: Descriptive study. Fish: A Total of 120 Nile tilapia fish (Oreochromis niloticus) samples were collected from Lake Manzala, and drainage water at different localities. Procedures: Cd, Cu, and Pb concentrations residues within mid-dorsal muscle tissue, within gills, liver, and kidney were determined. Erythrocyte count, hemoglobin concentration, Packed Cell volume and other blood indices, as well as, total leukocyte count were measured. Biochemically, Alanine transaminase, Aspartate transaminase activities, total protein, creatinine, uric acid, lysozymes activity were estimated. GST gene expression was determined in the liver. Results: The results showed that Pb, Cu and Cd were bio accumulated at a higher level in the liver, kidney and gills of Nile tilapia fish (Oreochromis niloticus) from all sampling sites. The levels of the ALT and AST were increased, total protein and Albumin concentrations were decreased. Creatinine and uric acid were significantly (P≤ 0.05) increased in all groups (compared to the control group. Hematological parameters and lysozyme activity were decreased. Up regulation of the hepatic GST expression levels in Nile tilapia exposed to the heavy metals in comparison to the control value. Conclusion and clinical relevance: This study shows that lead, copper and cadmium were bio accumulated at higher concentration in liver, kidney, gills and muscles of Nile tilapia due to large industrial activities near locations of the sampling sites. Additionally, GST gene expression represents sensitive biomarker of aquatic pollution.
Highlights
Fish are mainly susceptible and severely affected by pollution as they couldn't avoid harmful influences of pollutants because of feeding and existing within aquatic system [1]
Heavy metal pollution caused by direct atmospheric accumulation, geologic weathering or via the discharge of agricultural, municipal, residential or industrial waste products, by WWTPs [2]
A total of 120 Nile tilapia fish were collected from all sampling sites
Summary
Fish are mainly susceptible and severely affected by pollution as they couldn't avoid harmful influences of pollutants because of feeding and existing within aquatic system [1]. Heavy metals are formed from different natural and anthropogenic sources [3]. Contami nation by heavy metals and metalloids i n water and sediment, if existing within high levels, is a significant danger due to their toxicity, durability, and deposition and biomagnification within food chain [5]. Fish are regarded to become most significant bio-monitors in the aquatic system for evaluation of heavy metal pollution [2], they provide many particular benefits in illustrating natural properties of aquatic systems and in evaluating variations to habitats [6]. Beside fishexist within the end of the aquatic foodchainand can store heavy metals and transfer it to human via food leading to different disorders [7]
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