Abstract
Objective: To investigate the effects of panax notoginseng saponins on mobilizing bone marrow mesenchymal stem cells after cerebral infarction so as to provide experimental basis for Huoxue Huayu Decoction (HXHYD) that is promoting the blood flow and removing blood stasis. Methods: Apart from the normal group, all of the remaining rats must survive the improved Zea-Longa middle cerebral artery occlusion (MCAO) modeling method; all standard rats were randomly divided into four groups. The first group designated as control group was administered with normal saline by gavage every day. The second, third and fourth groups designated as drug groups were administered respectively with Xuesaitong soft Capsule (ingredients dissolved in normal saline) in 20, 40 and 60 mg/ml body weight/day (low-dose group, mid-dose group and high-dose group). The treatment continued for 28 days. Each group was then divided into subgroups (each subgroup contains at least 8 rats) determined by time points (1st day 3rd day 7th day 14th day and 28th day). Changes of CD54, CD106, CD105, CD117 in blood and bone marrow of rats were detected by FCM. The SCF level at different time points were determined though ELISA methods. Results: The stem cell factor (SCF) level in peripheral blood and bone marrow showed a significant increase in the control group and the treated group. The SCF in peripheral blood and bone marrow increased on the 1st day and reached a peak on the 14th day, then decreased in the peripheral blood gradually. The SCF level in peripheral blood showed a better increase in both medium-dose and high-dose groups than that of model group (p 0.05) between the lowdose group and the control group at each time point. Conclusion: PNS can mobilize the BMSCs to improve the levels in the peripheral blood.
Highlights
As one of the main resource of endogenous stem cells as well as the ideal seed cells for gene and cell therapy, the bone-marrow mesenchymal stem cells (BMSCs) represent a type of multi-potent cells with non-immunogenic and favorable tissue merging capacity
Blood stem cell factor (SCF) level in model groups began to lift from the 1st day and reached peak at the 14th day
Li Yong Hua [14] used components of Yangxin Tongmai Prescription to mobilize the BMSCs of rats with acute myocardial infarction(MI) and the results shows that compared with control group, a significant increase of CD34+ cells in peripheral blood in drug groups was found and the CD34+ cells in the cytoplasm of edge area of MI was increased
Summary
158 healthy SPF grade adult male Sprague-Dawley (SD) rats (280~300g) which purchased from Beijing Vital River Company were used in this research. Rabbit antibody to CD105, Rabbit polyclonal antibodies to rat CD105 and CD117 kits were purchased from Bioss (Bioss, Beijing). Precision balance (BS250S) was purchased from Saiduo LiSi Company, Beijing. After 2 hours of MCAO, withdrawing the intraluminal filament until a slight resistance was felt It indicated that the tip of nylon thread was back to the ICA and the reperfusion was accomplished. The second, third and fourth groups designated as drug groups were respectively administered with Xuesaitong soft Capsule (ingredients dissolved in normal saline) of 20, 40 and 60 mg/ kg body weight/day (low-dose group, mid-dose group and high-dose group). Plasma was measured with ELISA kits and blood for FCM, respectively. The levels of SCF in blood and bone marrow were measured with ELISA kits respectively.
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