Effect of platelet-rich plasma on wnt3 gene and klotho gene of adipose-derived stem cells of rabbit

Abstract

Objective To investigate the effect of platelet-rich plasma (PRP) on starting of wnt3 gene and klotho gene of adipose-derived stem cells (ADSCs) of rabbit. Methods Epididymal adipose tissue stem cells were obtained from New Zealand white rabbits, and the cells identified by morphology and inducing differentiation, and the cells were cultured to the fourth generation, PRP and PPP (platelet-poor plasma) were prepared by traditional centrifugal method from abdominal aortic of rabbit; ADSCs were cultured in culture medium containing PRP (experimental group), PPP (control group) and all medium (blank group) for each 5% for 24h, 48h and 72h. Cells of each group were dissociated and total RNA extracted. Effects of the starting of wnt3 gene and klotho gene were detected by RT-PCR. Results Primary ADSCs of rabbit grew in the way of long spindle swirly. The results of oil red O and alizarin red staining of the ADSCs were positive. Expression of wnt3 gene and klotho gene in the experimental group significantly increased from the results of RT-PCR (P<0.05). Conclusions PRP can promote proliferation of the ADSCs of rabbit and increase the expression of wnt3 gene and klotho gene significantly. Key words: Platelet-rich plasma; Adipose-derived stem cells; Wnt3 gene; Klotho gene