Abstract
Testicular damage is a serious complication of diabetes mellitus resulting in male infertility, which is associated with caspase-3 and IGF-1 mRNA expression. Platelet-rich plasma (PRP), with its rich growth factor composition, has proven beneficial in regenerative therapy. It is believed that PRP has not been studied in testes for diabetes mellitus and there are no studies in the literature concerning the influence of PRP on expressions of growth factors in testes. The aim of this study was to investigate the efficacy of adjunctive PRP in insulin treatment for repair of testicular damage in a diabetic rat model. Diabetes was induced by administering single dose 60 mg/kg streptozotocin. Twenty Wistar male rats were divided into four groups: group 1, control group; group 2, diabetes without treatment; group 3, diabetes with treated insulin; and group 4, diabetes with treated insulin and PRP. Rats were euthanized after two weeks of treatment, and testes were taken for caspase-3 and IGF-1 mRNA expression measurements. Diabetes mellitus induction caused a significant increase in caspase-3 mRNA expression with p =0.049 and significant decrease in IGF-1 mRNA expression with p =0.004. There was no difference in caspase-3 and IGF-1 mRNA expression of the diabetic rat testis given insulin and PRP compared to without PRP.
Highlights
Male reproductive system damage due to diabetes mellitus (DM) is a disorder that results in a decrease in male fertility (Kianifard et al, 2012; Shi et al, 2017; Sisman et al, 2014)
This study aimed to investigate the efficacy of adjunctive Platelet-rich plasma (PRP) in insulin treatment for repair of testicular damage in a diabetic rat model by assessing caspase-3 and IGF1 mRNA expression
Animal model-body weight and serum glucose Weight loss in the DM group compared to control group was significantly different (Table I)
Summary
Male reproductive system damage due to diabetes mellitus (DM) is a disorder that results in a decrease in male fertility (Kianifard et al, 2012; Shi et al, 2017; Sisman et al, 2014). Testicular damage due to DM is damage that occurs other than the pancreas (Sisman et al, 2014). A molecular mechanism in the form of oxidative stress and apoptosis causes testicular damage (Shi et al, 2017). Information about the mechanism of damage caused by DM through oxidative stress and apoptosis is the reason for further research, namely study on substances that can prevent oxidative stress and apoptosis and aid in repairing testicular damage due to DM. The increase in caspase-3 is a link between oxidative stress and apoptosis. The increase in caspase-3 in the testes becomes a measure of testicular damage and a decrease in caspase-3 is an indicator of the success of therapy (Morsy et al, 2014; Shi et al, 2017)
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