Abstract
Elimination of platelets from guinea pig splenocyte suspension (lacking megakaryocytes) with EDTA considerable reduces the efficiency cloning of splenic stromal precursor cells. It means that platelet-derived growth factors are necessary for stromal precursor cells from different organs (bone marrow, thymus, and spleen). The dependence on the platelet growth factors can vary within a wide range in descendants from cultured bone marrow precursor cells (passaged bone marrow fibroblasts at different stages of differentiation.
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