Effect of platelet automesoconcentrate on the immunological status of experimental animals after liver resection

Abstract

Objective. To study the indices of cellular and humoral immunity in the blood of rats after liver resection and under conditions of administration of platelet automeso–concentrate.
 Materials and methods. The study was performed on albino Wistar rats weighing 140 – 245 g. Resection of 2/3 of the liver was performed using the method of aseptic removal of its left and central lobes. During the resection, platelet autologous concentrate at a dose of 1 ml/kg was injected into the liver residue. To characterise the cellular and humoral components of immunity, the levels of leukocytes, lymphocytes, the number of total T– and B–lymphocytes, the level of circulating immune complexes, and the content of immunoglobulins of classes G and M were determined.
 Results. In the initial stages after liver resection, a marked leukocytosis was observed, accompanied by an increase in the relative number of basophils, eosinophils, rods and monocytes and a decrease in the level of segmented neutrophils and lymphocytes. The level of lymphocytes decreased at the expense of the T–cell population. Against the background of an increase in the number of B–lymphocytes, there was an increase in the levels of immunoglobulins M, G and circulating immune complexes. The injection of platelet autologous concentrate into the liver residue during resection of this organ stimulated the cellular and humoral immunity, as the studied parameters were higher than those of animals that did not receive platelet autologous concentrate. On the 7th day after partial hepatectomy, the studied parameters approached the control values.
 Conclusions. Components of cellular and humoral immunity can directly or indirectly affect the processes of liver regeneration after liver resection. B–cells after liver resection as antigen–presenting cells can trigger and modulate the immune response, which is enhanced by the introduction of platelet autologous concentrate into the body.