Abstract
Saccharomyces cerevisiae cultures were selected to determine the effects of plasmid size on host growth kinetics and plasmid copy number. A complete synthetic medium was verified and the effects of yeast nitrogen base without amino acids medium and leucine selection were established for the strain. The dependence of copy number on the plasmid size, medium, and oxygen availability was also measured. This study was part of a comprehensive effort to elucidate the behavior of recombinant yeast.
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