Abstract
Phalaenopsis orchids are one of the most beautiful flowering plants. The objective of this study was to identify the best plant growth regulator combination and medium for the growth and direct shoot formation from leaf explants of the hybrid Phalaenopsis ‘Pink’. Leaf tips segments from in vitro young plants were cultured on half-strength Murashige and Skoog (MS) and Vacin and Went (VW) media supplemented with different concentrations of auxin [α-napthaleneacetic acid (NAA)] and cytokinins [6-benzylaminopurine (BAP) or thidiazuron (TDZ)]. The explants that were cultured on 0 mg l–1 NAA and 3 mg l–1 BAP supplemented to half-strength MS medium formed shoots successfully within 10 weeks of culture with 5 % regenerants and 50 % survival frequency. The explants cultured on 0.5 mg l–1 NAA and 1.5 mg l–1 TDZ supplemented to half-strength MS medium developed calluses and shoots within 11 weeks of culture with 25 % regenerants and 90 % survival frequency. Future research needs to be directed to find out the shortest time of shoot regeneration to produce viable plants with a high survival frequency.
Highlights
Species from the genus Phalaenopsis, Orchidaceae, known as “moth orchids”, are one of the most popular, beautiful and unique flowering plants in the world due to their large, colourful, durable flowers and their adaptability to room condition
The combination of napthaleneacetic acid (NAA) and BAP supplemented to half-strength Murashige and Skoog (MS) medium significantly affected the growth and direct shoot formation of leaf explants (Table 1)
Shoots directly formed at 0.5 mg l–1 NAA and 2 mg l–1 BAP within 10–11 weeks of culture with 40 % survival frequency
Summary
Species from the genus Phalaenopsis, Orchidaceae, known as “moth orchids”, are one of the most popular, beautiful and unique flowering plants in the world due to their large, colourful, durable flowers and their adaptability to room condition. They have a very high economic value in Europe, Asia and other continents. They are commercially grown for the production of the cut flowers and potted plants (Košir et al, 2004; Niknejad et al, 2011). The characteristics of seedlings propagated by ordinary vegetative means are not uniform and propagation through tissue culture has been the most desired way of propagation (Košir et al, 2004)
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