Effect of Plant Growth Regulators and Additives on Indirect Organogenesis of Simarouba glauca DC

Abstract

A protocol of in vitro propagation of Simarouba glauca DC (Simaroubaceae) by indirect organogenesis under the influence of different combinations of plant growth regulators (PGRs) was standardized. Indole-3-butyric acid 1.0 mg/l produced the highest callusing response: 100 % in cotyledon and 95 % in internode explant. One hundred percent of shoot bud induction response with shoot number 18.0 for cotyledon and 85 % response of shoot bud induction with shoot number 12.5 for internode were observed in 6-benzyl adenine (BA) 3.0 mg/l, 6-furfuryl amino purine 0.5 mg/l, and GA31.0 mg/l. Organic additive coconut water that resulted in 95 % response with average 25.0 shoot number for cotyledon and 80 % response with average shoot number of 18.2 for internode was observed in the medium containing CW (15 %). GA3 promoted only shoot multiplication with no shoot elongation. High concentration of GA3 directly involved in shoot elongation rather multiplication, but it caused some irreversible root inhibition. α-Naphthalene acetic acid 2.0 mg/l showed 95 % and 80 % of rooting response with root numbers 8.5 and 7.2 in cotyledon and internode explants, respectively. The microshoots with the lengthy roots were transferred to paper cups for acclimatization. After a period of 12 weeks, acclimatized plants were successfully transferred to the field. The 56.8 % plants survived in the field.