EFFECT OF PLACENTA PLASMA ON THE SULFATE EXCHANGE IN TISSUES

Abstract

Recently, Dziewiatkowski, Bostrom and others (1) studied on the metabolism of S35 labelled sodium sulfate in vitro and in vivo. They found that, when a tracer dose of S35 as sulfate is given to an experimental animal, most of the radioactive sulfate is rapidly excreted, only a small fraction being retained in the tissues, mainly cartilage, bone and bone marrow and that most of the radioactive sulfur in these tissues are deposited as ester sulfate in chondroitinsulfuric acid.It is well known (2) that cortisone inhibits the formation of granulation tissue which has a synthetic capacity of chondroitinsulfuric acid. Layton (3) and Bostrom et al. (4) demonstrated by use of isotope tracer method that cortisone inhibited the synthesis of chondroitinsulfuric acid in embryonic and wound tissues.In our institute, it was found that placenta plasma (5), suspension of cold-stored human placenta in a normal saline solution, was remarkably efficacious to the proliferative diseases of connective tissue. It may be, therefore, inevitably necessary to investigate the effect of placenta plasma on the metabolism of chondroitinsulfuric acid in connective tissue.In this work, the authors studied on the effect of placenta plasma and cortisone on the utilization of S35 labelled sulfate by various tissues of mice and on the elimination of radioactive sulfur from their tissues. And the difference of the effect on sulfate exchange between them was investigated.