Effect of PKCα on the gene expression related with proliferation and activity of transcription factor AP-1

Abstract

PROTEIN kinase C (PKC) is a serine/thronine kinase, which plays a major role in cell signalling, growth control and tumorigenesis. PKC is encoded by a multigene family, which have been identified as consisting of at least 12 members in mammalian tissues. PKC isoforms differ in their biochemical properties, tissue-specific expression and intracellular localization. The differences imply that the individual PKC isoforrns have a distinct physiological function:']. In order to study the role of specific PKC subtype in cell proliferation and transformation, we constructed the human embryonic lung cell (2BS) model stably overexpressing PKCa and found that the overexpression of PKCa promoted the growth rateL2] and caused the transforming phenotype in 2BS cells[". Usually, the extracellular signals act on the cellular phenotype through influence on the gene expression in nucleus. So far, little is known about the molecular mechanism of the role of PKCa in the control of cell growth. In the present study, we observed the effect of overexpression of PKCa on gene expression of CaM, cyclinBl and early response genes related with proliferation and activity of transcription factor AP-1.