Effect of pituitary-specific transcription factory small interfering RNA on biological behaviors of growth hormone pituitary adenoma GH3 cells in rats

Abstract

Objective To observe the effects of pituitary-specific transcription factory (Pit-1)small interfering RNA (siRNA) on biological behaviors of growth hormone pituitary adenoma GH3 cells in rats.Methods Design and synthesis of the Pit-1 gene of siRNA,mixed with liposome transfection of GH3 cells.Using protein immunoblot assay (Western blotting),reverse transcription polymerase chain reaction (RT-PCR) assay for the determination,after siRNA translated 24 h,of Pit-1 protein and Pit-1 mRNA expression ; with cell counting kit-8 (CCK-8) cell proliferation-toxicity test in the detection of cell growth increment change; by flow cytometry for detection of apoptosis changes ; system preparation of Transwell cell determination of GH3 cells in vitro invasion ability changes.Results SiRNA significantly inhibited Pit-1 protein and the Pit-1 mRNA the expression level decreased by 58.18％ and 75.82％,respectively;CCK-8 method results showed that the siRNA group cell growth significantly lower than the negative control group; siRNA group increased apoptosis rate (9.250 00 ±0.996 95)％ compared with the negative control group (2.893 30±0.665 16)％ ;siRNA group through the Matrigel gum and cell membrane (92.91 ± 53.25)months,with the negative control group (7.50 ± 7.95) comparison invasion force significantly weakened,and the difference was statistically significant (P ＜0.05).Conclusion Pit-1 siRNA can effectively inhibit GH3 cell growth,promote their apoptosis,reduced invasiveness. Key words: Pituitary adenomas; Small interfering RNA; Biological behavior