Effect of pioglitazone on the growth of normal bladder transitional epithelial cells and bladder cancer cells

Abstract

The normal bladder transitional cells and bladder cancer J82 cells were treated with various concentrations of pioglitazone for 24 h,48 h,72 h,8 d,and 10 d.The growth of the cells was tested by MTT.Apoptosis rate was detected by flow cytometry technology.Realtime-PCR and Western blot were used to analyze the mRNA and protein expressions of p53,cvclin D1,Bcl-2,and Bax.lhe results showed that the cell viability of normal bladder cells was decreased 24,48,and 72 h after 10 μmol/L pioglitazone treatment (all P＜0.05) while the cell apoptosiswasincreased [24h(18.8±2.1 vs 9.4 ± 1.7) ％,48 h (29.9 ± 1.3 vs 1 0.7 ± 1.1),72 h (49.7 ±2.3 vs11.3 ± 1.3) ％,P＜0.05].Pioglitazone had no effect on the growth and proliferation of J82 cells.There were no significant differences in mRNA and protein expressions of p53,cvclin D1,Bcl-2,and Bax within 72 h after pioglitazone treatment in this two kinds of cells.But,the protein expressions of cvclin D1 and p53 in J82 cells were decreased after pioglitazone treatment for 8 days.These results suggest that pioglitazone does not increase the risk of bladder cancer in normal transitional cells. Key words: PPARγ; Thiazolidinediones; Pioglitazone; Bladder cancer