Effect of Pingchuanguben Decoction on interleukin-17 of bronchial alveolar lavage fluid and airway inflammation in mice with asthma

Abstract

Objective To study the effect of Pingchuanguben Decoction on IL-17 level of bronchial alveolar lavage fluid (BALF) and airway remolding in mice with asthma. Methods Forty healthy female Kunming mice were randomly divided into 4 groups (10 rats in each group): normal control group, asthma group, Pingchuanguben Decoction group and Budesonide group.The asthmatic models were established by ovalbumin (OVA); the normal control group was treated with phosphate buffer solution (PBS liquid) by celiac injection, during this period Pingchuanguben Decoction was administered to the mice in Pingchuanguben Decoction group each day.In stimulation stage, the normal control group was atomization inhalated PBS liquid, and the rest of the 3 groups were given aerosolized OVA instead.Bude-sonide atomization was administered to mice in Budesonide group before stimulation.The levels of IL-17 in BALF were measured with enzyme-linked immunosorbent assay (ELISA), and the levels of matrix metalloproteinase-9 (MMP-9) and the tissue inhibitor of metalloproteinase-1(TIMP-1) in lung tissues were tested with immunohistochemical analysis. Results 1.The levels of IL-17 in BALF in Pingchuanguben Decoction group and Budesonide group were significantly lower than those in asthma group (all P 0.05). Conclusions Pingchuanguben Decoction may restrain airway inflammation and remolding, which can replace Budesonide to a certain extent. Key words: Asthma; Interleukin-17; Matrix metalloproteinase-9; Pingchuanguben Decoction; Budesonide suspension; Mouse