Effect of Phytohormones of Kinetin and Epibrassinolide on Content and Intracellular Localization of Glucosides and Free Amino Acids in Pea Plants Cells (Pisum sativum L.)

Abstract

During injection of kinetin and epibrassinolide in pea seedlings (Pisum sativum L.) an increase of content of specific for this compound isosuccinimide-β-glucoside (IS-glucoside) for 15-30%, ethyl-β-glucoside for 40-80%, GABA free amino acids, glutamate and alanine only under kinetin for 15-20% was observed. The field experiments showed an increase of aglycone content but the amount of IS-glucoside decreased at first and then increased 14 days after plant treatment with phytohormones. It was shown that effect of phytohormones on content of IS-glucosides and free amino acids was dependent on action time and age of plant. By using membrane trophic compound of DMSO it was discovered that in pea seedlings cells the vacuolar fund of IS-glucoside was 70% of initial and aglycone with γ-aminobutyric acid as its precursor were localized in cytoplasm. Aspartate and glutamate were almost equally distributed between cytoplasmic and vacuolar cell funds. It is suggested that reactions of pyrrolidone structure of IS-glucoside aglycone formation and synthesis of the glucoside were processed in cytoplasm. Further IS-glucoside was transferred and stored in vacuoles of pea seedlings cells by contrast with ethyl-β-glucoside mainly localized in cytoplasm.