Effect of Phytochrome Deficit on Activity of Ascorbate Peroxidase and Phenylalanine Ammonia-Lyase and Expression of Genes APX1, tAPX, sAPX, and PAL in the Leaves of Arabidopsis thaliana Plants Exposed to UV-A and Red Light

Abstract

Effect of UV-A radiation (10 W/m2, 2 h) and modifying effect of brief exposure to red light (RL) (1.8 W/m2, 20 min) on photochemical activity of photosystem II (PSII) (Fv/Fm), as well as on activity of ascorbate peroxidase (AsP) and phenylalanine ammonia-lyase (PAL), were investigated in the leaves of 26–28-day-old Аrabidopsis thaliana (L.) Heynh. plants of wild type (WT) and mutants: hy3 deficient in phytochrome B (phyB) and hy2 defective in phytochromobilin synthesis. UV-A reduced photochemical activity of PSII and activities of AsP and PAL. Exposure to RL increased PAL activity in only WT plants and in mutant hy3. Elevation of expression of genes APX1, sAPX, tAPX, and PAL encoding enzymes AsP and PAL was detected upon the exposure to RL and UV-A. For instance, expression of gene APX1 in WT plants and in mutant hy3 rose upon exposure to RL and UV-A; in mutant hy2, it increased only upon UV-A treatment. Expression of gene tAPX in WT plants rose only under the effect of RL, whereas expression of gene sAPX did not rise in any type of treatment. In mutant hy2, expression of the above-named genes decreased upon the effect of RL. Upon joint action of RL and UV-A (RL followed by UV), the negative effect of UV-A on the activity of the total pool of AsP was nullified by RL in only WT plants and in mutant hy3. Partial removal of the negative effect of UV-A on activity of PSII as a result of pretreatment of plants with RL is probably related to a rise in the activity of antioxidant enzymes. This is evident in WT plants whose PSII is more sensitive to RL radiation than in mutants deficient in phytochromes. We believe that PSII resistance to UV-A radiation depends on the potential of the plant antioxidant system, which rises when the share of the active form of phytochrome in its total pool increases as a result of plants’ pretreatment with RL and is partially realized by way of induction of transcriptional activity of antioxidant genes.