Abstract
Phytic acid (PA) could increase the yellowness value of yellow alkaline noodles (YAN), as well as significantly reduce the generation of dark spots on the surface of YAN by suppressing polyphenol oxidase (PPO) browning. In this paper, the inhibition mechanism of PA on PPO browning was mainly studied. In the produced YAN sheets, the PPO activity and free quinone content showed a gradual decrease with increasing PA addition, while the free phenol content gradually increased. This indicated that PA could inhibit PPO browning and was an effective browning inhibitor. In the constructed in vitro PPO solution, the anti-PPO browning mechanism of PA was mainly through chelating Cu2+ in the active center. The DPPH radical scavenging rate of PA was measured, and it was found that PA could inhibit PPO browning, which was related to the antioxidant capacity. The effect of PA on browning products was analyzed using HPLC, and the results of the study showed that PA could reduce the peak area of the browning products, thus reducing the generation of dark spots.
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