Effect of phycocyanin on expression of malondialdehyde and interferon-γ placental trophoblast cells models of preeclampsia rats

Abstract

Preeclampsia is a "disease of theory" because of the cause and pathophysiology of preeclampsia is still uncertain. In preeclampsia invasion of trophoblast cells does not occur completely, the spiral arteries not undergo remodeling so it still has a component of muscle and elastic tissue, in addition to endothelial dysfunction characterized by reduced relaxation factor and increased contraction factor resulting in ischemia of the placenta by The end result of hypoxia, hypoxia is the condition that causes the production of free radicals in large quantities. Free radicals can damage all the cellular biochemical components of lipids by taking lipid electrons in a cell membrane called lipid peroxidation. Lipid peroxidation process produces several products including Malondialdehyde (MDA) also mediate proinflammatory cytokines such expenditure Interferon Gamma (IFN-γ). In this study, researchers took the form of placental tissue paraffin blocks which have been made, on each of the paraffin block containing a number of 6 groups of placental tissue (30 blocks of paraffin). The method used is double staining with the reading of 3 field trophoblast cells with 200x magnification. On examination Malondialdehyde done by TBARS and analyzed by immunoflourence by staining rhodamine and DAPI, while on inspection of IFN-γ of the preparations that has been staining with antibodies PE anti-rat IFN-γ, and DAPI were analyzed using immunoflourence, then interpreted using software imunoflow. Provision of Phycocyanin in this study proved to reduce levels of MDA and Interferon-γ. Phycocyanin dose 40 ng / 100 gramss BB which has an effective dose decreases MDA and Interferon-γ levels, Because of phycocyanin has a role as an anti-inflammatory, antioxidant and immunomodulatory. In giving of Phycocyanin which in large doses can trigger an increase in proinflammatory cytokines. Further research is needed on the protective effect of Phycocyanin on human placental tissue.