Effect of photosensitizer concentration and treatment time on fluorescence diagnosis

Abstract

Objective To investigate the effect of photosensitizer concentration and treatment time on blue-ray induced fluorescence diagnosisi,in order to improve the accuracy of fluorescence diagnosis.Methods Tumor-bearing mice were prepared in regular way,intravenous injection were given with different concentrations (0～70 mg/kg) of hematoporphyrin derivative (HpD) as photosensitizer,at different time after drug administration (0～48 h),iodine gallium lamp with pre-filter of 395～415 nm was used as light source,blue-ray induced fluorescence was obtained.Fluorescence images were collected by image acquisition system,and biopsy histopathology slices were obtained.Results HpD concentration was 40 mg/kg o body weight,after 18 hours administration,the range of tumor infiltration could be recognized more accurately,with higher fluorescence diagnostic accuracy and lower false positive rate compaired with other experiment groups.Statistical differences was significant.Conclusion Photosensitizer concentration and treatment time are closely related to the effect of fluorescence diagnosis,optimization of these two parameters can improve the accuracy of fluorescence diagnosis and reduce the false positive rate,which can provide a reference for tumor surgery program. Key words: Fluorescence diagnosis; Carrier fluorescence; Photosensitizer; Hematoporphyrin derivative