Effect of phosphorus concentration on the photochemical stability of PSII and CO2 assimilation in Pistacia vera L. and Pistacia atlantica Desf.

Abstract

A greenhouse pot experiment was conducted at the faculty of sciences of Gafsa to evaluate the effect of phosphorus treatment on two pistachio species. The seedlings of Pistacia vera and Pistacia atlantica were subjected to six levels of phosphoric acid (P2O5) (0, 5, 15, 30, 60 and 120 ppm). Stomatal conductance, net photosynthesis, chlorophyll fluorescence (OJIP) and total chlorophyll content were measured after 1, 2, 3, 6, 8, 9 and 12 weeks of treatment. During the experiment, phosphorus application at 5 ppm increased photosynthesis and stomatal conductance, relative to the treatment 0 ppm only in P. atlantica. However, phosphorus supply at 60 and 120 ppm induced toxicity leading to an inhibition of CO2 photo-assimilation rate, an alteration of photosystem II (PSII) structure and function and reduction in leaf chlorophyll content in both species. The (OJIP) transient showed complex changes in O-J, J-I and I–P phases of fluorescence. Due to phosphorus toxicity, both donor and acceptor sides of PSII were damaged, electron transport perturbed and chlorophyll pigment reduced which resulted in the fall of CO2 photo-assimilation rate, followed by mortality in both species.